The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system
Summary
SNTA1 encodes α1-syntrophin, a scaffold protein, which is a component of the dystrophin-associated protein complex. Additionally, α1-syntrophin interacts with SCN5A and nNOS-PMCA4b complex in cardiomyocytes. SNTA1 is a susceptibility locus for arrhythmia and cardiomyopathy. We generated a homozygous SNTA1 knockout human embryonic stem cell (H9SNTA1KO) using the CRISPR/Cas9 system. H9SNTA1KO maintained pluripotency and a normal karyotype and differentiated into three germ layers in vivo. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Dong T, Zhang S, Chang Y, Bai R, Jiang Y, Ma S, Li Y, Jiang HF, Lu WJ |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Mar;51:102196 |
| PubMed | 33524674 |
| DOI | 10.1016/j.scr.2021.102196 |