The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system

Summary

SNTA1 encodes α1-syntrophin, a scaffold protein, which is a component of the dystrophin-associated protein complex. Additionally, α1-syntrophin interacts with SCN5A and nNOS-PMCA4b complex in cardiomyocytes. SNTA1 is a susceptibility locus for arrhythmia and cardiomyopathy. We generated a homozygous SNTA1 knockout human embryonic stem cell (H9SNTA1KO) using the CRISPR/Cas9 system. H9SNTA1KO maintained pluripotency and a normal karyotype and differentiated into three germ layers in vivo. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Dong T, Zhang S, Chang Y, Bai R, Jiang Y, Ma S, Li Y, Jiang HF, Lu WJ
Journal Stem cell research
Publication Date 2021 Jan 26;51:102196
PubMed 33524674
DOI 10.1016/j.scr.2021.102196

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