Generating dHAND homozygous knockout human embryonic stem cell line (WAe009-A-59) by episomal vector-based CRISPR/Cas9 system


In order to determine the function of dHAND in a specific subset of cardiomyocyte progenitor cells responsible for the heart and its function in the cellular mechanism of Hand2-/- mouse ventricular hypoplasia, we established an allelic knockout model of dHAND in human embryonic stem cells (hESCs-H9) by an episomal vector-based CRISPR/Cas9 system. This dHAND KO hESC line maintained normal karyotype and typical primed pluripotent human stem cell morphology, and maintained pluripotency, could differentiate into all three germ layers in vivo. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Wang W, Bai R, Song X
Journal Stem cell research
Publication Date 2021 Aug;55:102471
PubMed 34332328
DOI 10.1016/j.scr.2021.102471

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