Generation of a homozygous MYH7 gene knockout human embryonic stem cell line (WAe009-A-69) using an episomal vector-based CRISPR/Cas9 system

Summary

Myosin heavy chain 7 (MYH7) encodes the human heart myosin heavy chain subunit, which plays an important role in myocardial contraction. MYH7 is the main pathogenic gene that causes Hypertrophic cardiomyopathy (HCM) and Dilated cardiomyopathy (DCM). In this experiment, a MYH7 homozygous knockout human embryonic stem cell (hESC) line, WAe009-A-69, was generated using an episomal vector-based CRISPR/Cas9 system. It can be an ideal tool to further study the function of MYH7. The cell line was confirmed with pluripotency, normal karyotype and trilineage differentiation potential. Copyright © 2021. Published by Elsevier B.V.

Authors Guo T, Jiang Y, Song Y, Ma S, Chang Y, Zhang S, Wang H, Dong T, Jiang H, Lu W
Journal Stem cell research
Publication Date 2021 Oct 11;57:102566
PubMed 34678661
DOI 10.1016/j.scr.2021.102566

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