Understanding the role of transposable elements in establishing gene regulatory networks during early embryonic lineage commitment and segregation
|Title||Understanding the role of transposable elements in establishing gene regulatory networks during early embryonic lineage commitment and segregation|
|Sponsor||Horizon 2020 (H2020)|
|Institution||Institute of Molecular Biotechnology|
|Principal investigator||Nicolas Rivron
Associated cell lines
Embryonic development begins with a single fertilized cell, leading to a complete body plan following a series of morphogenetic events. Core genetic components such as transcription factors and signalling molecules exhibit a high level of conservation across vertebrates. However, comparative studies have highlighted characteristic species-specific variations during pre-implantation development even within closely related species such as human, mouse and marmoset. These studies suggested that even though a sequence of embryology appears similar, morphogenetic events differ significantly due to differences in underlying molecular mechanisms establishing species-specific gene regulatory networks (GRNs) that remain elusive due to inaccessibility to amenable embryo models. Here, I propose to take advantage of the newly developed three-dimensional human blastoid system and integrate the power of genetic manipulations and high throughput sequencing technologies to understand mechanisms by which GRNs are established and regulated during pre-and post implantation development. Our proposed study will have a special emphasis on characterizing the role of regulatory sequences derived from transposable elements and mechanisms employed by the embryonic system to utilize them for precise lineage segregation.