HNES-1, HNES1

General#

Cell Line

hPSCreg Name CAMe001-A
Alternative name(s)
HNES-1, HNES1
Cell line type Human embryonic stem cell (hESC)
Last update 4th December 2018
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Provider

Generator University of Cambridge (CAM)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_9R98

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2014-09-11
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Medium Other medium:
Base medium: DMEM/F12 and Neurobasal (1:1)
Main protein source: Bovine serum albumin
Serum concentration: %
Supplements
B27 10 ml
N2 5 ml
Insulin 1 µg/ml
L-glutamine 2 mM
2-mercaptoethanol 0.1 mM
PD0325901 1 µM
CHIR99021 1 µM
Y-27632 10 µM
Human LIF 10 ng/ml
Ascorbic acid 250 µM

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Unkown
SSEA-4
Unkown
TRA 1-60
Unkown
TRA 1-81
Unkown
ZFP42 (REX-1)
Unkown
KLF4
Yes
TFCP2L1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)