HNES-1, HNES1

General#

Cell Line

hPSCreg Name CAMe001-A
Alternative name(s)
HNES-1, HNES1
Cell line type Human embryonic stem cell (hESC)
Last update 4th December 2018
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Provider

Generator University of Cambridge (CAM)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_9R98

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Ethics#

Was the embryo established purely for research purposes? Yes
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number

hESC Derivation#

Date of derivation 2014-09-11
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Medium Other medium:
Base medium: DMEM/F12 and Neurobasal (1:1)
Main protein source: Bovine serum albumin
Serum concentration: %
Supplements
B27 10 ml
N2 5 ml
Insulin 1 µg/ml
L-glutamine 2 mM
2-mercaptoethanol 0.1 mM
PD0325901 1 µM
CHIR99021 1 µM
Y-27632 10 µM
Human LIF 10 ng/ml
Ascorbic acid 250 µM

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Unkown
SSEA-4
Unkown
TRA 1-60
Unkown
TRA 1-81
Unkown
ZFP42 (REX-1)
Unkown
KLF4
Yes
TFCP2L1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)