Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23)

Summary

Pituitary homeobox 3 (Pitx3) is a key transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons. Here, we established a PITX3-mCherry knock-in reporter human embryonic stem cell (hESC) line using the CRISPR/Cas9 system. PITX3-mCherry hESCs maintained pluripotency marker expression and exhibited the capacity to generate all 3 germ layers and a normal karyotype. After differentiation into mDA neurons, most PITX3 immunoreactivity overlapped with the red fluorescence of mCherry. This reporter cell line may be used to study the development of mDA neurons or to enrich mDA populations for transplantation. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Park S, Yoo JE, Lee DR, Jang J, Cho MS, Kim DS, Kim DW
Journal Stem cell research
Publication Date 2019 Jul 18;39:101499
PubMed 31352199
DOI 10.1016/j.scr.2019.101499

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