H9-CRISPRi

General

Cell Line

hPSCreg name WAe009-A-2C
Cite as:
WAe009-A-2C
Alternative name(s)
H9-CRISPRi
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 17th May 2024
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Provider

Generator University of Copenhagen (KU)
Derivation country Denmark

External Databases

BioSamples SAMEA115622335

General Information

Projects
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • None at all
  • NONE
  • None
  • No abnormalities related to cardiovascular diseases
show more synonyms
Genetic modifications not related to a disease
AAVS1 (Adeno-Associated Virus Integration Site 1) (target)
Gene knock-in
19q13
CRISPRi in AAVS1 locus
CRISPR-associated (CRISPR/Cas) System