Generation of a TLR7 heterozygous knockout line (WAe009-A-18) from human embryonic stem cells using CRISPR/Cas9
Toll-like receptor 7 (TLR7) is a member of the toll-like receptor (TLR) family that is essential in the innate immune system. In this study, we established a heterozygous TLR7 knockout H9 cell line using CRISPR/Cas9. TLR7 knockout H9 cells maintained their pluripotency and exhibited the ability to differentiate into the three germ layers without any karyotype abnormalities. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Seo HH, Han HW, Kim JH|
|Journal||Stem cell research|
|Publication Date||2020 May;45:101735|