Generation of a TLR7 heterozygous knockout line (WAe009-A-18) from human embryonic stem cells using CRISPR/Cas9
Summary
Toll-like receptor 7 (TLR7) is a member of the toll-like receptor (TLR) family that is essential in the innate immune system. In this study, we established a heterozygous TLR7 knockout H9 cell line using CRISPR/Cas9. TLR7 knockout H9 cells maintained their pluripotency and exhibited the ability to differentiate into the three germ layers without any karyotype abnormalities. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Seo HH, Han HW, Kim JH |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 May;45:101735 |
| PubMed | 32268246 |
| DOI | 10.1016/j.scr.2020.101735 |