Generation of a TRPM8 knockout hESC line (WAe009-A-A) derived from H9 using CRISPR/Cas9
Summary
The transient receptor potential cation channel subfamily M member 8 (TRPM8) is a kind of non-selective cation channel which controls Ca2+ homeostasis. Mutations in TRPM8 were related to dry eye diseases (DED). Here we constructed a TRPM8 knockout cell line WAe009-A-A from the original embryonic stem cell line H9 using CRISPR/Cas9 technology, which maybe helpful for exploring the pathogenesis of DED. WAe009-A-A cells possess stem cell morphology and pluripotency as well as normal karyotype, and have the ability of differentiating into three germ layers in vitro. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Bai J, Zhang J, Fu W, Li S, Tian X, Li X, Zhao X, Dong J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Mar;67:103040 |
| PubMed | 36796252 |
| DOI | 10.1016/j.scr.2023.103040 |