Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4(G1003D) Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)
Summary
The chromatin remodeler Chromodomain-helicase-DNA-binding protein 4 (CHD4) is crucial for the development of multiple organ systems. Functional mutations of CHD4 have recently been described in a developmental disorder, namely Siffrim-Hitz-Weiss syndrome (SIHIWES). Herein, we have generated a homozygous CHD4G1003D hESC line (WAe025-A-1) using CRISPR/eCas9-based gene editing in the WA-25 hESC line. The edited hESC line maintains normal karyotype, pluripotency, and ability to differentiate into three germ layers. This cell line will be a valuable resource for studying the functional role of CHD4 during the development and disease modeling of SIHIWES in vitro.
| Authors | Chohra I, Giri S, Malgrange B |
|---|---|
| Journal | International journal of molecular sciences |
| Publication Date | 2023 Jun 23;24(13) |
| PubMed | 37445725 |
| PubMed Central | PMC10342000 |
| DOI | 10.3390/ijms241310543 |