WAe009-A-2Z

H9-CRISPRi

The cell line is not validated yet.

General

Cell Line

hPSCreg name WAe009-A-2Z
Cite as:
WAe009-A-2Z
Alternative name(s)
H9-CRISPRi
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 20th January 2025
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Provider

Generator Helmholtz Zentrum München (HMGU)
Owner Helmholtz Zentrum München (HMGU)

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzymatically
EDTA
Medium Essential 8™ Flex
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
pHAGE EF1α dCas9-KRAB
CRISPR-associated (CRISPR/Cas) System