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ICANi001-A-1

Registration Summary:

CDGEN1.16.40.5

ICANi001-A-1

General

Cell Line
hPSCreg name ICANi001-A-1
Cite as:
ICANi001-A-1 (RRID:CVCL_A5RF)
Alternative name(s)
CDGEN1.16.40.5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
ICANi001-A
(CDGEN1.16)
Donor diseases:
hypertrophic cardiomyopathy
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IBBISTi006-A
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Last update 1st October 2021
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Provider
Generator INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Owner INSERM U1166-Institute of Cardiometabolism And Nutrition (ICAN)
Derivation country France

External Databases
BioSamples SAMEA10203005
Cellosaurus CVCL_A5RF
Wikidata Q107115416

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Hypertrophic cardiomyopathy
The donor is a carrier of a disease-associated mutation and affected.
Stage
NYHA 4
Synonyms
  • Asymmetric Septal Hypertrophies
  • Hypertrophic Obstructive Cardiomyopathies
  • Cardiomyopathy, hypertrophic
  • Subvalvular Stenosis, Idiopathic Hypertrophic
  • Hypertrophic Obstructive Cardiomyopathy
  • HYPERTR OBSTR CARDIOMYOP
  • hyper. obst. cardiomyopathy
  • SUBVALV STENOSIS
  • HCM - Hypertrophic cardiomyopathy
  • IDIOPATHIC HYPERTROPHIC SUBVALV STENOSIS
  • Obstructive Cardiomyopathy, Hypertrophic
  • Hypertrophies, Asymmetric Septal
  • Hypertrophic Cardiomyopathies
  • Cardiomyopathies, Hypertrophic Obstructive
  • Hypertrophic cardiomyopathy (disorder)
  • HOCM - Hypertrophic obstructive cardiomyopathy
  • Primary hypertrophic cardiomyopathy
  • Idiopathic Hypertrophic Subvalvular Stenosis
  • IHSS
  • primary hypertrophic cardiomyopathy (disorder) [Ambiguous]
  • Idiopathic Hypertrophic Subaortic Stenosis
  • Obstructive cardiomyopathy
  • SUBVALV STENOSIS IDIOPATHIC HYPERTROPHIC
  • Hypertrophy, Asymmetric Septal
  • Hypertrophic obstructive cardiomyopathy (disorder)
  • Septal Hypertrophy, Asymmetric
  • Cardiomyopathies, Hypertrophic
  • IHSSs
  • hypertrophic myocardiopathy
  • Cardiomyopathy, Hypertrophic Obstructive
  • HCM
  • Obstructive Cardiomyopathies, Hypertrophic
  • Asymmetric Septal Hypertrophy
  • Septal Hypertrophies, Asymmetric
  • hypertrophic subaortic stenosis
  • obstructive hypertrophic cardiomyopathy
  • hypertrophic obstructive cardiomyopathy
  • hypertrophic cardiomyopathy
  • familial hypertrophic cardiomyopathy
  • HCM - hypertrophic cardiomyopathy
show more synonyms
Genetic variants
14q11.2
NM_000257.4:c.1208G>T
NP_000248.2:p.Arg403Leu
Heterozygous
VCV000014101.6
rs121913624

Karyotyping (Donor)
Has the donor karyotype been analysed?
Unknown

External Databases (Donor)
BioSamples SAMEA12775561

Ethics

Also have a look at the ethics information for the parental line ICANi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line ICANi001-A.

Reprogramming method
Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Alkaline Phosphatase
Yes
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
Karyotype CDGEN1-16-40.5
Karyotype_CDGEN1-16-40_5.JPG
Passage number: 20
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Hypertrophic cardiomyopathy
Synonyms
  • Asymmetric Septal Hypertrophies
  • Hypertrophic Obstructive Cardiomyopathies
  • Cardiomyopathy, hypertrophic
  • Subvalvular Stenosis, Idiopathic Hypertrophic
  • Hypertrophic Obstructive Cardiomyopathy
  • HYPERTR OBSTR CARDIOMYOP
  • hyper. obst. cardiomyopathy
  • SUBVALV STENOSIS
  • HCM - Hypertrophic cardiomyopathy
  • IDIOPATHIC HYPERTROPHIC SUBVALV STENOSIS
  • Obstructive Cardiomyopathy, Hypertrophic
  • Hypertrophies, Asymmetric Septal
  • Hypertrophic Cardiomyopathies
  • Cardiomyopathies, Hypertrophic Obstructive
  • Hypertrophic cardiomyopathy (disorder)
  • HOCM - Hypertrophic obstructive cardiomyopathy
  • Primary hypertrophic cardiomyopathy
  • Idiopathic Hypertrophic Subvalvular Stenosis
  • IHSS
  • primary hypertrophic cardiomyopathy (disorder) [Ambiguous]
  • Idiopathic Hypertrophic Subaortic Stenosis
  • Obstructive cardiomyopathy
  • SUBVALV STENOSIS IDIOPATHIC HYPERTROPHIC
  • Hypertrophy, Asymmetric Septal
  • Hypertrophic obstructive cardiomyopathy (disorder)
  • Septal Hypertrophy, Asymmetric
  • Cardiomyopathies, Hypertrophic
  • IHSSs
  • hypertrophic myocardiopathy
  • Cardiomyopathy, Hypertrophic Obstructive
  • HCM
  • Obstructive Cardiomyopathies, Hypertrophic
  • Asymmetric Septal Hypertrophy
  • Septal Hypertrophies, Asymmetric
  • hypertrophic subaortic stenosis
  • obstructive hypertrophic cardiomyopathy
  • hypertrophic obstructive cardiomyopathy
  • hypertrophic cardiomyopathy
  • familial hypertrophic cardiomyopathy
  • HCM - hypertrophic cardiomyopathy
show more synonyms
Genetic modifications
MYH7 (target)
Variant
14q11.2
NM_000257.4:c.1208T>G
NP_000248.2:p.Leu403Arg
Heterozygous
VCV000014101.6
rs121913624
the modification is a reversion of the heterozygous mutation R403L, both alleles are WT (R403).
Genetic modifications not related to a disease
MYH7 (target)
Gene knock-in
14q11.2
14q11.2
The recombination matrix (ssODN) introduced to deliver the R403 reversion also carried a silent P402P snp. NM_000257.4:c.1206A>G
CRISPR-associated (CRISPR/Cas) System