Bi-allelic mutant, Mut 103
UKEi070-A-2
General
Cell Line |
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| hPSCreg name | UKEi070-A-2 |
| Cite as: | UKEi070-A-2 (RRID:CVCL_A0YU) |
| Alternative name(s) |
Bi-allelic mutant, Mut 103
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
SCVIi074-A (SCVIi599C1) Donor's gene variants: BAG cochaperone 3 Donor diseases: Hypertrophic Cardiomyopathy |
| Last update | 26th May 2021 |
| User feedback | |
Provider |
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| Generator | University Medical Center Hamburg-Eppendorf (UKE) |
| Owner | University Medical Center Hamburg-Eppendorf (UKE) |
External Databases |
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| BioSamples | SAMEA8944461 |
| Cellosaurus | CVCL_A0YU |
| Wikidata | Q108821442 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: Cardiovascular Research
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
MTA |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
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External Databases (Donor) |
|
| BioSamples | SAMEA8944456 |
Ethics
Also have a look at the ethics information for the parental line
UKEi070-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UKEi070-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| O2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: DMEM F12
Main protein source: Human Serum Albumin Serum concentration: 0 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| TNNT2 troponin T2, cardiac type [ Homo sapiens (human) ] |
Yes |
In vitro directed differentiation
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XX
Karyotyping method:
Nanostring nCounter Karyotype panel
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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