General

Cell Line
hPSCreg name	ICGi029-A-2
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	ICGi029-A-2
Alternative name(s)	HCM14-3wt8
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	24th October 2024
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Provider
Generator	Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG) Contact: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Owner	Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Distributors	Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Derivation country	Russia
External Databases
BioSamples	SAMEA116305255
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	ICGi029-A

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Hypertrophic Cardiomyopathy The donor is affected. Synonyms Hypertrophic Cardiomyopathy
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
External Databases (Donor)
BioSamples	SAMEA8307097

Ethics

Also have a look at the ethics information for the parental line ICGi029-A .

Is there an MTA available for the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?	sgRNA (GenScript), Cas9_NLS (NEB), and single-stranded donor oligonucleotides (Biolegio) were used for correction of the c.1543_1545delAAC (p.N515del) mutation in the MYBPC3 gene of patient-specific iPSCs (the ICGi029-A line)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above?	No

hIPSC Derivation

General
The source cell information can be found in the parental cell line ICGi029-A.
Passage number reprogrammed	1
Reprogramming method
Vector type	Non-integrating
Vector	Episomal
Genes	POU5F1 SOX2 KLF4 MYCL LIN28 Trp53
Is reprogramming vector detectable?	No
Methods used	PCR
Vector free reprogramming
Other
Selection criteria for clones	ESC-like morphology
Derived under xeno-free conditions	No
Derived under GMP?	No
Available as clinical grade?	No

Culture Conditions

Surface coating	Gelatin
Feeder cells	Mouse embryonic fibroblasts
Passage method	Enzymatically TrypLE
O2 Concentration	20 %
CO2 Concentration	5 %
Medium	Other medium: Base medium: Knock-out DMEM Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements Supplement Amount Unit NEAA 0.1 mM GlutaMAX 1 mM penicillin 100 U/ml streptomycin 100 µg/ml 2-mercaptoethanol 0.05 mM bFGF 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
POU5F1 (OCT-4)	Yes	–
SOX2	Yes	–	–
NANOG	Yes		–
SSEA-4	Yes	–

Morphology

Morphology pictures

ICGi029-A-2_Morphology.tif

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Marker	Expressed
KRT18	Yes

Morphology

ICGi029-A-2_CK18.tif

Mesoderm

Smooth Muscle Cell
Ont Id: CL_0000192

In vitro spontaneous differentiation

Marker	Expressed
ACTA2	Yes

Morphology

ICGi029-A-2_aSMA.tif

Ectoderm

Neuron
Ont Id: CL_0000540

In vitro spontaneous differentiation

Marker	Expressed
TUBB3	Yes

Morphology

ICGi029-A-2_TUBB3.tif

Microbiology / Virus Screening
Mycoplasma	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46,XX ICGi029-A-2_karyotype.jpg Passage number: 8 Karyotyping method: G-Banding
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

Hypertrophic Cardiomyopathy Synonyms Hypertrophic Cardiomyopathy Genetic modifications MYBPC3 (target) Type of modification Isogenic modification Chromosome location 11p11.2 Nucleotide sequence variant in HGVS format NM_000256.3:c.1543_1545delAAC Protein sequence variant in HGVS format NP_000247.2:p.Asn515del Is the modification homozygous or heterozygous? Heterozygous Free text A trinucleotide c.1543_1545delAAC (p.N515del) deletion was corrected in exon 17 of the MYBPC3 gene in patient-specific iPSCs (the ICGi029-A line) using CRISPR/Cas9 and single-stranded donor oligonucleotides How has the target locus been modified? Repaired Available documents MYBPC3-pN515del_correction.tif

Genetic modifications not related to a disease

Type of modification Isogenic modification Chromosome location 11p11.2 Is the modification homozygous or heterozygous? Heterozygous Free text Two synonymous substitutions were introduced in exon 17 of the MYBPC3 gene of patient-specific iPSCs (the ICGi029-A line) using CRISPR/Cas9 and single-stranded donor oligonucleotides to modify PAM and disrupt a restriction site for the BclI restriction endonuclease How has the target locus been modified? Mutated