CBIGi001-A-1

PRKN-KO, PRKN-KO/AIW002-02

General

Cell Line

hPSCreg Name
CBIGi001-A-1
Alternative name(s)
PRKN-KO, PRKN-KO/AIW002-02
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Last update 7th February 2022
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG)
Owner Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG)
Distributors
Derivation country Canada

External Databases

BioSamples SAMEA12565661
CLO CLO_0103734
Cellosaurus CVCL_C0JZ
Wikidata Q112929367

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12565539

Ethics

Also have a look at the ethics information for the parental line CBIGi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line CBIGi001-A.

Reprogramming method

Vector type None

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Parkinson's Disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic modifications
PRKN (target)
Gene knock-out
6
The CBIGi001-A-1 line was generated by knocking out PRKN in CBIGi0001-A. Knock out of PRKN1 confirmed by ddPCR and Sanger sequencing.
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease