Generation of homozygous PRKN, PINK1 and double PINK1/PRKN knockout cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing
Summary
Autosomal recessive mutations in either PRKN or PINK1 are associated with early-onset Parkinson's disease. The corresponding proteins, PRKN, an E3 ubiquitin ligase, and the mitochondrial serine/threonine-protein kinase PINK1 play a role in mitochondrial quality control. Using CRISPR/CAS9 technology we generated three human iPSC lines from the well characterized AIW002-02 control line. These isogenic iPSCs contain homozygous knockouts of PRKN (PRKN-KO, CBIGi001-A-1), PINK1 (PINK1-KO, CBIGi001-A-2) or both PINK1 and PRKN (PINK1-KO/PRKN-KO, CBIGi001-A-3). The knockout lines display normal karyotypes, express pluripotency markers and upon differentiation into relevant brain cells or midbrain organoids may be valuable tools to model Parkinson's disease. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Chen CX, You Z, Abdian N, Sirois J, Shlaifer I, Tabatabaei M, Boivin MN, Gaborieau L, Karamchandani J, Beitel LK, Fon EA, Durcan TM |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Jul;62:102806 |
| PubMed | 35561458 |
| DOI | 10.1016/j.scr.2022.102806 |