PINK1-KO, PINK1-KO/AIW002-02
CBIGi001-A-2
General
Cell Line |
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| hPSCreg name | CBIGi001-A-2 |
| Cite as: | CBIGi001-A-2 (RRID:CVCL_C0K0) |
| Alternative name(s) |
PINK1-KO, PINK1-KO/AIW002-02
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
CBIGi001-A-3 (PINK1-KO/PRKN-KO/AIW002-02, PINK1-KO/PRKN-KO) CBIGi001-A (AIW002-02) CBIGi001-A-1 (PRKN-KO, PRKN-KO/AIW002-02) |
| Last update | 7th February 2022 |
| User feedback | |
Provider |
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| Generator | Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG) |
External Databases |
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| BioSamples | SAMEA12866579 |
| Cellosaurus | CVCL_C0K0 |
| Wikidata | Q112929368 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA12565539 |
Ethics
Also have a look at the ethics information for the parental line
CBIGi001-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
CBIGi001-A.
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
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| Medium |
mTeSR™ 1
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| SSEA-4 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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