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CBIGi001-A-2

Registration Summary:

PINK1-KO, PINK1-KO/AIW002-02, IPSC0072

CBIGi001-A-2

General

Cell Line
hPSCreg name CBIGi001-A-2
Cite as:
CBIGi001-A-2 (RRID:CVCL_C0K0)
Alternative name(s)
PINK1-KO, PINK1-KO/AIW002-02, IPSC0072
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
CBIGi001-A-3
(PINK1-KO/PRKN-KO/AIW002-02, PINK1-KO/PRKN-KO, IPSC0069)
CBIGi001-A
(AIW002-02, IPSC0063)
CBIGi001-A-1
(PRKN-KO, PRKN-KO/AIW002-02, IPSC0070)
CBIGi001-A-4
(MAPT P301L/AIW002-02, IPSC0065)
CBIGi001-A-5
(MAPT-KO/AIW002-02, IPSC0066)
CBIGi001-A-8
(IPSC0071, PINK1-3flag/AIW002-02)
CBIGi001-A-9
(IPSC0073, PRKN A401D/AIW002-02)
CBIGi001-A-10
(IPSC0074, PRKN W403A/AIW002-02)
CBIGi001-A-11
(IPSC0075, PRKN Y143D/AIW002-02)
CBIGi001-A-12
(PRKN-V393D/AIW002-02, IPSC0076)
CBIGi001-A-13
(IPSC0078, SCARB2-KO/AIW002-02)
CBIGi001-A-14
(IPSC0079, SH3GL2-KO/AIW002-02)
CBIGi001-A-15
(SNCA A30P/AIW002-02, IPSC0080)
CBIGi001-A-16
(IPSC0081, SNCA A53T/AIW002-02)
CBIGi001-A-17
(IPSC0082, SNCA E46K/AIW002-02)
CBIGi001-A-18
(IPSC0083, SNCA-KO/AIW002-02)
CBIGi001-A-21
(IPSC0086, SMPD-KO/AIW002-02)
CBIGi001-A-24
(TMEM163-KO/AIW002-02, IPSC0096)
CBIGi001-A-25
(IPSC0097, TMEM175-KO/AIW002-02)
CBIGi001-A-26
(USP19-KO/AIW002-02, IPSC0098)
CBIGi001-A-28
(IPSC0100, ASAH1-KO/AIW002-02)
CBIGi001-A-30
(BIN3-KO/AIW002-02, IPSC0102)
CBIGi001-A-33
(CLCN3-KO/AIW002-02, IPSC0105)
CBIGi001-A-34
(CTSB-KO/AIW002-02, IPSC0106)
CBIGi001-A-35
(FAM49B(CYRIB)-KO/AIW002-02, IPSC0107)
CBIGi001-A-39
(IPSC0111, GALC-KO/AIW002-02)
CBIGi001-A-40
(IPSC0112, GBA-KO/AIW002-02)
CBIGi001-A-41
(IPSC0113, GBAP1_promoter-KO/AIW002-02)
CBIGi001-A-42
(IGSF9B-KO/AIW002-02, IPSC0114)
CBIGi001-A-43
(INPP5F-KO/AIW002-02, IPSC0115)
CBIGi001-A-44
(IP6K2-KO/AIW002-02, IPSC0116)
CBIGi001-A-45
(LRRK2-KO/AIW002-02, IPSC0117)
CBIGi001-A-7
(IPSC0068, OPTN-KO/AIW002-02)
CBIGi001-A-19
(IPSC0084, SOD1 G93A/AIW002-02)
CBIGi001-A-22
(TARDBP-A382T/AIW002-02, IPSC0089)
CBIGi001-A-23
(TARDBP-G348C/AIW002-02, IPSC0090)
CBIGi001-A-29
(ATAXIN3-KO/AIW002-02, IPSC0101)
CBIGi001-A-31
(IPSC0103, C9ORF72-KO/AIW002-02)
CBIGi001-A-37
(IPSC0109, FUS-H517Q/AIW002-02)
CBIGi001-A-38
(IPSC0110, FUS-KO/AIW002-02)
BCHi019-A-7
(HNDS0179-01#B KO32(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-7
(HNDS0141-01#B KO7(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-8
(HNDS0141-01#B KO14(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-1
(HNDS0141-01#B CNC22(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-2
(HNDS0141-01#B CNC35(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-3
(HNDS0141-01#B CC6(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-4
(HNDS0141-01#B CC43(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-5
(HNDS0141-01#B CC8(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-6
(HNDS0141-01#B CC23(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
CBIGi001-A-6
(IPSC0067, OFD1-KO/AIW002-02)
Last update 11th March 2025
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Provider
Generator Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG)

External Databases
BioSamples SAMEA12866579
Cellosaurus CVCL_C0K0
Wikidata Q112929368

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA12565539

Ethics

Also have a look at the ethics information for the parental line CBIGi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line CBIGi001-A.

Reprogramming method
Vector type None

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Parkinson Disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic modifications
PTEN induced kinase 1 (target)
Gene knock-out
Chromosome 1
The CBIGi001-A-2 line was generated by knocking out PINK1 in CBIGi0001-A. Knock out of PINK1 confirmed by ddPCR and Sanger sequencing.
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease