CBIGi001-A-2

PINK1-KO, PINK1-KO/AIW002-02

General#

Cell Line

hPSCreg Name
CBIGi001-A-2
Alternative name(s)
PINK1-KO, PINK1-KO/AIW002-02
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 7th February 2022
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Provider

Generator Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG)

External Databases

BioSamples SAMEA12866579
CLO CLO_0103735

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12565539

Ethics#

Also have a look at the ethics information for the parental line CBIGi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line CBIGi001-A.

Reprogramming method

Vector type None

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Parkinson's Disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic modifications
PTEN induced kinase 1 (target)
Gene knock-out
Chromosome 1
The CBIGi001-A-2 line was generated by knocking out PINK1 in CBIGi0001-A. Knock out of PINK1 confirmed by ddPCR and Sanger sequencing.
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease