hPSCreg®
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CBIGi001-A-3

Registration Summary:

PINK1-KO/PRKN-KO/AIW002-02, PINK1-KO/PRKN-KO

CBIGi001-A-3

General

Cell Line
hPSCreg name CBIGi001-A-3
Cite as:
CBIGi001-A-3 (RRID:CVCL_C0K1)
Alternative name(s)
PINK1-KO/PRKN-KO/AIW002-02, PINK1-KO/PRKN-KO
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
CBIGi001-A-1
(PRKN-KO, PRKN-KO/AIW002-02)
CBIGi001-A-2
(PINK1-KO, PINK1-KO/AIW002-02)
CBIGi001-A
(AIW002-02)
BCHi014-A-1
(HNDS0141-01#B CNC22(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-2
(HNDS0141-01#B CNC35(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-3
(HNDS0141-01#B CC6(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-4
(HNDS0141-01#B CC43(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-5
(HNDS0141-01#B CC8(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi014-A-6
(HNDS0141-01#B CC22(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-1
(HNDS0164-01#D CNC9(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-2
(HNDS0164-01#D CNC11(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-3
(HNDS0164-01#D CC2(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-4
(HNDS0164-01#D CC16(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-5
(HNDS0164-01#D CC3(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi017-A-6
(HNDS0164-01#D CC10(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-1
(HNDS0176-01#A CNC10(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-2
(HNDS0176-01#A CNC35(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-3
(HNDS0176-01#A CC3(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-4
(HNDS0176-01#A CC16(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-5
(HNDS0176-01#A CC38(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi018-A-6
(HNDS0176-01#A CC48(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-1
(HNDS0179-01#B CNC2(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-2
(HNDS0179-01#B CNC21(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-3
(HNDS0179-01#B CC13(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-4
(HNDS0179-01#B CC15(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi019-A-5
(HNDS0179-01#B CC26(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi020-A-1
(HNDS0163-01#B CNC20(+/-))
Donor diseases:
PTEN Hamartoma Tumor Syndrome
BCHi020-A-2
(HNDS0163-01#B CC19(+/+))
Donor diseases:
PTEN Hamartoma Tumor Syndrome
BCHi020-A-3
(HNDS0163-01#B CC34(+/+))
Donor diseases:
PTEN Hamartoma Tumor Syndrome
BCHi020-A-4
(HNDS0163-01#B CC12(-/-))
Donor diseases:
PTEN Hamartoma Tumor Syndrome
BCHi013-A-1
(HNDS0143-01#A CNC21(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi013-A-2
(HNDS0143-01#A CNC24(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi013-A-3
(HNDS0143-01#A CC12(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi013-A-4
(HNDS0143-01#A CC28(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-1
(HNDS0138-01#B CNC24(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-2
(HNDS0138-01#B CNC42(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-3
(HNDS0138-01#B CC38(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-4
(HNDS0138-01#B CC51(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-5
(HNDS0138-01#B CC6(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi015-A-6
(HNDS0138-01#B CC11(-/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi016-A-1
(HNDS0151-01#B CNC20(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi016-A-2
(HNDS0151-01#B CNC43(+/-))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi016-A-3
(HNDS0151-01#B CC18(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
BCHi016-A-4
(HNDS0151-01#B CC23(+/+))
Donor diseases:
PTEN hamartoma tumor syndrome
LCSBi012-A-1
(RHOT1_T610A_clone62.19.37_IsogenicControl)
Donor diseases:
Parkinson's Disease
SUSMi005-A-1
(SNCA3X 0KO C1, SNCA3X 0KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-2
(SNCA3X 1KO C2, SNCA3X 1KO C1)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-3
(SNCA3X 2KO C1, SNCA3X 2KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-4
(SNCA3X 3KO C1, SNCA3X 3KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-5
(SNCA3X 4KO C1, SNCA3X 4KO C2)
Donor diseases:
obsolete_Parkinson's disease
Last update 7th February 2022
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Provider
Generator Clinical Biospecimen Imaging and Genetic (C-BIG) Repository (CBIG)

External Databases
BioSamples SAMEA12866580
Cellosaurus CVCL_C0K1
Wikidata Q112929369

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases No disease was diagnosed.

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)
BioSamples SAMEA12565539

Ethics

Also have a look at the ethics information for the parental line CBIGi001-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line CBIGi001-A.

Reprogramming method
Vector type None

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Parkinson's Disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic modifications
PRKN (target)
Gene knock-out
Chromosome 6
See cell line CBIGi0001-A-1. Knock out of PRKN confirmed by ddPCR and Sanger sequencing.
CRISPR-associated (CRISPR/Cas) System
PTEN induced kinase 1 (target)
Gene knock-out
Chromosome 1
The CBIGi001-A-3 line was generated by knocking out PINK1 in CBIGi0001-A-1 (PRKN-KO) line. Knock out of PINK1 confirmed by ddPCR and Sanger sequencing.
CRISPR-associated (CRISPR/Cas) System