BIHi005-A-4
General#
Cell Line |
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| hPSCreg Name | BIHi005-A-4 |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 26th April 2018 |
| User feedback | |
Provider |
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| Generator |
Berlin Institute of Health (BIH)
Contact:
Berlin Institute of Health (BIH) |
| Owner | Berlin Institute of Health (BIH) |
| Distributors | |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA1009820 |
General Information |
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| Notes | SORT1 KO in BIHi005-A |
| Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | male |
| Ethnicity | asian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | Mutation in the LMNA gene in other family members but not in this sample |
Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has sibling:
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External Databases (Donor) |
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| BioSamples | SAMEA104012190 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
BIHi005-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | |
| Is reprogramming vector detectable? |
Unkown |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
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| mRNA | |
Other |
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| Derived under xeno-free conditions |
Unkown |
| Derived under GMP? |
Unkown |
| Available as clinical grade? |
Unkown |
Culture Conditions#
| Medium |
Essential 8™
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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Genotyping#
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification#
| Disease/phenotype related modifications |
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