BIHi005-A-4
General
Cell Line |
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| hPSCreg name | BIHi005-A-4 |
| Cite as: | BIHi005-A-4 |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
| Last update | 22nd May 2019 |
| Notes | SORT1 KO in BIHi005-A |
| User feedback | |
Provider |
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| Generator |
Berlin Institute of Health (BIH)
Contact:
Berlin Institute of Health (BIH) |
| Owner | Berlin Institute of Health (BIH) |
| Distributors | |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA1009820 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | asian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | Mutation in the LMNA gene in other family members but not in this sample frame shift mutation in the LMNA gene that leads to the early termination of translation (c. 349_350insG; p. K117fs) |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has brother:
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External Databases (Donor) |
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| BioSamples | SAMEA104012190 |
Ethics
Also have a look at the ethics information for the parental line
BIHi005-A
.
| Alternatives to consent | |
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Constraints for use or distribution |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
BIHi005-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
|
| mRNA | |
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Essential 8™
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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Genotyping
Karyotyping (Cell Line) |
|
Other Genotyping (Cell Line) |
Genetic Modification
| Disease/phenotype related modifications |
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