BIHi005-A-4
General#
Cell Line |
|
| hPSCreg Name | BIHi005-A-4 |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Berlin Institute of Health (BIH)
Contact:
Berlin Institute of Health (BIH) |
| Owner | Berlin Institute of Health (BIH) |
| Distributors | |
| Derivation country | Germany |
External Databases |
|
| BioSamples | SAMEA1009820 |
| CLO | CLO_0102750 |
General Information |
|
| Notes | SORT1 KO in BIHi005-A |
| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: not allowed
Commercial: not allowed
|
| Subclone of | |
Donor Information#
General Donor Information |
|
| Sex | male |
| Ethnicity | asian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | Mutation in the LMNA gene in other family members but not in this sample |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has brother:
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External Databases (Donor) |
|
| BioSamples | SAMEA104012190 |
Ethics#
Also have a look at the ethics information for the parental line
BIHi005-A
.
| Alternatives to consent | |
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Constraints for use or distribution |
hIPSC Derivation#
General |
|
|
The source cell information can be found in the parental cell line
BIHi005-A.
|
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Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
mRNA
|
| mRNA | |
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
| Medium |
Essential 8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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Genotyping#
Karyotyping (Cell Line) |
|
Other Genotyping (Cell Line) |
Genetic Modification#
| Disease/phenotype related modifications |
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