BIHi005-A-86
General
iPSC Line |
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| hPSCreg name | BIHi005-A-86 |
| Cite as: | BIHi005-A-86 |
| iPSC line type | Human induced pluripotent stem cell (hiPSC) |
| Similar iPSC lines |
BIHi005-A-87 (hiPSCKITD816V-87) ESIBIe003-A-8 (KIT D816V ESC 1) ESIBIe003-A-9 (KIT D816V ESC 2) BIHi049-A (SCVI-112) |
| Last update | 2nd April 2025 |
| User feedback | |
Provider |
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| Generator |
Berlin Institute of Health (BIH)
Contact:
Berlin Institute of Health (BIH) |
| Owner | Berlin Institute of Health (BIH) |
| Distributors | |
| Derivation country | Germany |
External iPSC Databases |
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| BioSamples | SAMEA117690970 |
General iPSC Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
iPSC Genetic Modification
| Disease/phenotype related modifications |
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Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | asian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | Mutation in the LMNA gene in other family members but not in this sample frame shift mutation in the LMNA gene that leads to the early termination of translation (c. 349_350insG; p. K117fs) |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has brother:
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External Databases (Donor) |
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| BioSamples | SAMEA104012190 |
Ethics
Also have a look at the ethics information for the parental line
BIHi005-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
iPSC Derivation
General |
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The source cell information can be found in the parental cell line
BIHi005-A.
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
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| mRNA | |
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
iPSC Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this iPSC line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this iPSC line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this iPSC line? | Yes |
iPSC Characterisation
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
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| Is there a certificate of analysis available? |
Yes
Passage:
28
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Genotyping
Karyotyping (iPSC Line) |
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| Has the iPSC line karyotype been analysed? |
Yes
46, XY (cp20)
Passage number: 28
Karyotyping method:
G-Banding
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Other Genotyping (iPSC Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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