ESIBIe003-A-8

KIT D816V ESC 1

General#

Cell Line

hPSCreg Name
ESIBIe003-A-8
Alternative name(s)
KIT D816V ESC 1
Cell line type Human embryonic stem cell (hESC)
Last update 11th March 2021
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Provider

Generator Universitätsklinikum Aachen (UKA)
Owner Universitätsklinikum Aachen (UKA)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA8359801
CLO CLO_0103212

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104620499

Ethics#

Also have a look at the ethics information for the parental line ESIBIe003-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation#

The source cell information can be found in the parental cell line ESIBIe003-A.

Culture Conditions#

Surface coating Gelatin
Feeder cells mouse embryonic fibroblast
Passage method Enzymatically
Collagenase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knock out DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
bFGF 10 ng/ml
non-essential amino acids 1 %
penicillin 100 U/ml
streptomycin 100 U/ml
L-glutamine 2 mM
b-mercaptoethanol 0.1 mM
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
systemic mastocytosis
Synonyms
  • SMCD - systemic mast cell disease
  • systemic tissue mast cell disease
Genetic modifications
KIT (target)
Variant
4q12
NM_000222.3:c.2447A>T
NP_000213.1:p.Asp816Val
Heterozygous
VCV000013852.5