ESIBIe003-A

HES-3, hES 3

General#

Cell Line

hPSCreg Name ESIBIe003-A
Alternative name(s)
HES-3, hES 3
Cell line type Human embryonic stem cell (hESC)
Last update 22nd November 2017
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Provider

Generator ES Cell International Pte Ltd. (ESIBI)
Derivation country Singapore

External Databases

Cellosaurus CVCL_7158

General Information

Publications
Projects
Is the cell line available in principle?
Yes
Subclones

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104620499

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
FGF 2 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GCTM2
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
Alkaline Phosphatase
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)