ESIBIe003-A

HES-3, hES 3

General#

Cell Line

hPSCreg Name ESIBIe003-A
Alternative name(s)
HES-3, hES 3
Cell line type Human embryonic stem cell (hESC)
Last update 8th January 2020
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Provider

Generator ES Cell International Pte Ltd. (ESIBI)
Derivation country Singapore

External Databases

Cellosaurus CVCL_7158

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Subclones

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104620499

Ethics#

Was the embryo established purely for research purposes? Yes
Have both parents consented to the use of the embryo for ESC derivation? No
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Brandi Roberts, 1010 Atlantic Ave #102, Alameda, CA 94501, +1(510) 521-3390
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? Yes
Contact information / weblink Brandi Roberts, 1010 Atlantic Ave #102, Alameda, CA 94501, +1(510) 521-3390
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? Yes
Alternatives to consent A local ethics committee at the University Leiden provided consent to use the line for the proposed research, including its derivatives.
Alternative consent approval number The line was NIH registered until 2009 and not resubmited afterwards
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
an academic institution? Yes
How may genetic information associated with the cell line be accessed?

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically and Mechanically
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
FGF 2 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GCTM2
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
Alkaline Phosphatase
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)