HES-3, hES 3
ESIBIe003-A
General#
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | |
Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA104620499 |
Ethics#
Alternatives to consent are available? | Yes |
Alternatives to consent |
Name of accrediting authority involved? |
Approval number |
hESC Derivation#
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
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PGD Embryo? |
No |
ZP removal technique | Enzymatic |
Cell isolation | Immunosurgery |
Cell seeding | Isolated ICM |
Culture Conditions#
Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
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Passage method | Enzymatically and Mechanically | ||||||
Medium |
Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
GCTM2 |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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DNMT3B |
Yes |
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GABRB3 |
Yes |
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GDF3 |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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ZFP42 (REX-1) |
Yes |
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SOX2 |
Yes |
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TDGF1 |
Yes |
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Alkaline Phosphatase |
Yes |
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TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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