hES3.3
ESIBIe003-A-3
General#
Cell Line |
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hPSCreg Name | ESIBIe003-A-3 |
Alternative name(s) |
hES3.3
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 13th September 2017 |
User feedback | |
Provider |
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Generator |
ES Cell International Pte Ltd. (ESIBI)
Contact:
hES cell provision |
Derivation country | Australia |
External Databases |
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Cellosaurus | CVCL_7161 |
General Information |
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Publications | |
Is the cell line available in principle? |
Yes |
Subclone of |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | |
Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA104620499 |
Ethics#
We are currently revising this section.hESC Derivation#
Supernumerary embryos from IVF treatment? |
Yes
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Culture Conditions#
Passage method | Enzymatic, Mechanical | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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Alkaline Phosphatase |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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