hES3.3
ESIBIe003-A-3
General#
Cell Line |
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| hPSCreg Name | ESIBIe003-A-3 |
| Alternative name(s) |
hES3.3
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 13th September 2017 |
| User feedback | |
Provider |
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| Generator |
ES Cell International Pte Ltd. (ESIBI)
Contact:
hES cell provision |
| Derivation country | Australia |
External Databases |
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| Cellosaurus | CVCL_7161 |
General Information |
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| Publications | |
| Is the cell line available in principle? |
Yes |
| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | |
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA104620499 |
Ethics#
We are currently revising this section.hESC Derivation#
| Supernumerary embryos from IVF treatment? |
Yes
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Culture Conditions#
| Passage method | Enzymatic, Mechanical | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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