ESIBIe003-A-6
General#
Cell Line |
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hPSCreg Name | ESIBIe003-A-6 |
Cell line type | Human embryonic stem cell (hESC) |
Last update | 20th January 2021 |
User feedback | |
Provider |
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Generator | Université Libre de Bruxelles (ULB) |
Owner | Université Libre de Bruxelles (ULB) |
Distributors | |
Derivation country | Belgium |
External Databases |
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BioSamples | SAMEA6724118 |
CLO | CLO_0102922 |
General Information |
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* Is the cell line readily obtainable for third parties? |
No |
Subclone of |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA104620499 |
Ethics#
Also have a look at the ethics information for the parental line
ESIBIe003-A
.
Is there an MTA available for the cell line? | Yes |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation#
Date of derivation | 2017-12-06 |
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
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PGD Embryo? |
No |
ZP removal technique | Enzymatic |
Cell isolation | Immunosurgery |
Cell seeding | Isolated ICM |
Culture Conditions#
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium: StemFlex
Main protein source: Serum concentration: % |
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
No characterisation data could be found for this subclone. Please open parental cell line ESIBIe003-AGenotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
Genetic Modification#
Genetic modifications not related to a disease |
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