ESIBIe003-A-6

General

Cell Line

hPSCreg name ESIBIe003-A-6
Cite as:
ESIBIe003-A-6 (RRID:CVCL_A5HA)
Cell line type Human embryonic stem cell (hESC)
Similar lines
ESIBIe003-A
(HES-3, hES 3)
ESIBIe003-A-11
(KIT+/- ESC)
ESIBIe003-A-8
(KIT D816V ESC 1)
ESIBIe003-A-12
(KIT-/- ESC)
ESIBIe003-A-9
(KIT D816V ESC 2)
ESIBIe003-A-7
(HES-3_44, IRF8-/- ES cell)
Last update 20th January 2021
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Provider

Generator Université Libre de Bruxelles (ULB)
Owner Université Libre de Bruxelles (ULB)
Distributors
Derivation country Belgium

External Databases

BioSamples SAMEA6724118
Cellosaurus CVCL_A5HA
Wikidata Q107114911

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104620499

Ethics

Also have a look at the ethics information for the parental line ESIBIe003-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line ESIBIe003-A.

Culture Conditions

Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line ESIBIe003-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 15
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
The original line was obtained from Andrew Elefanty (Monash University, australia- See MTA) and has a GFP cassette inserted in the NKX2.1 locus. In the present subcloned, a lentivirus was used to introduce a cassette combining two genes NKX2.1 and PAX8 fused by an IRES sequence. NKX2.1 : HGNC:11825 PAX8: HGNC:8622 Those two genes are under the control of a promoter inducible by doxycycline via a tetracycline response element.
Viral
Lentivirus