ESIBIe003-A-6
General#
Cell Line |
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| hPSCreg Name | ESIBIe003-A-6 |
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 20th January 2021 |
| User feedback | |
Provider |
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| Generator | Université Libre de Bruxelles (ULB) |
| Owner | Université Libre de Bruxelles (ULB) |
| Distributors | |
| Derivation country | Belgium |
External Databases |
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| BioSamples | SAMEA6724118 |
| CLO | CLO_0102922 |
| Cellosaurus | CVCL_A5HA |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA104620499 |
Ethics#
Also have a look at the ethics information for the parental line
ESIBIe003-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation#
| Date of derivation | 2017-12-06 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions#
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: StemFlex
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
No characterisation data could be found for this subclone. Please open parental cell line ESIBIe003-AGenotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Genetic modifications not related to a disease |
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