ESIBIe003-A-2

hES3.2

General#

Cell Line

hPSCreg Name ESIBIe003-A-2
Alternative name(s)
hES3.2
Cell line type Human embryonic stem cell (hESC)
Last update 13th September 2017
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Provider

Generator ES Cell International Pte Ltd. (ESIBI)
Derivation country Australia

External Databases

Cellosaurus CVCL_7160

General Information

Publications
Is the cell line available in principle?
Yes
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104620499

Ethics#

We are currently revising this section.

hESC Derivation#

Supernumerary embryos from IVF treatment?
Yes

Culture Conditions#

Feeder cells hFF (Human Foreskin Fibroblast)
Cellfinder Ont Id: CELDA_000001419
Passage method Enzymatic, Mechanical
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GATA6
Yes
NANOG
Yes
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)

Genetic Modification#