hES3.2
ESIBIe003-A-2
General
Cell Line |
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| hPSCreg name | ESIBIe003-A-2 |
| Cite as: | ESIBIe003-A-2 (RRID:CVCL_7160) |
| Alternative name(s) |
hES3.2
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
ESIBIe003-A (HES-3, hES 3) ESIBIe003-A-3 (hES3.3) ESIBIe003-A-4 (Envy) ESIBIe003-A-1 (hES3.1) ESIBIe003-A-11 (KIT+/- ESC) ESIBIe003-A-8 (KIT D816V ESC 1) ESIBIe003-A-12 (KIT-/- ESC) ESIBIe003-A-9 (KIT D816V ESC 2) ESIBIe003-A-7 (HES-3_44, IRF8-/- ES cell) |
| Last update | 13th September 2017 |
| User feedback | |
Provider |
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| Generator |
ES Cell International Pte Ltd. (ESIBI)
Contact:
hES cell provision |
| Derivation country | Australia |
External Databases |
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| Cellosaurus | CVCL_7160 |
| Wikidata | Q54882902 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA104620499 |
Ethics
Also have a look at the ethics information for the parental line
ESIBIe003-A
.
| Alternatives to consent |
hESC Derivation
|
The source cell information can be found in the parental cell line
ESIBIe003-A.
|
Culture Conditions
| Feeder cells |
hFF (Human Foreskin Fibroblast) Cellfinder Ont Id: CELDA_000001419 |
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| Passage method | Enzymatic, Mechanical | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| GATA6 |
Yes |
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| NANOG |
Yes |
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| Alkaline Phosphatase |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
|
Other Genotyping (Cell Line) |
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