ESIBIe003-A-10

General#

Cell Line

hPSCreg Name
ESIBIe003-A-10
Cell line type Human embryonic stem cell (hESC)
Last update 27th July 2021
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Provider

Generator Murdoch Children's Research Institute (MCRI)
Derivation country Australia

External Databases

BioSamples SAMEA9462434
CLO CLO_0103485

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104620499

Ethics#

Also have a look at the ethics information for the parental line ESIBIe003-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation#

Date of derivation 2014-08-27
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line ESIBIe003-A

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
INSULIN (target)
Gene knock-in
cytoband location 11p15.5
GFP was inserted into one allele of the INSULIN locus as described in Micallef et al 2012
Homologous Recombination
GLUCAGON (target)
Gene knock-in
cytoband location 2q24.2
mCherry was inserted into one allele of the GLUCAGON locus as described in Labonne et al 2021
TALEN