C5-PARK2-KO
The cell line is not validated yet.
GIBHi002-A-1
General
Cell Line |
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| hPSCreg name | GIBHi002-A-1 |
| Cite as: | GIBHi002-A-1 (RRID:CVCL_YC27) |
| Alternative name(s) |
C5-PARK2-KO
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
GIBHi002-A (C11) CBIGi007-A-1 (IPSC0009, TMEM175 Q65Q homozygous correction) Donor's gene variants: TMEM175, TMEM175 Donor diseases: Parkinson Disease CBIGi007-A-2 (IPSC0010, TMEM175 P65P homozygous disease) Donor's gene variants: TMEM175, TMEM175 Donor diseases: Parkinson Disease CBIGi009-A-1 (IPSC0013, TMEM175 M393T homozygous correction) Donor's gene variants: TMEM175, TMEM175 Donor diseases: Parkinson Disease CBIGi013-A-1 (LRRK2 M1646T correction, IPSC0018) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi027-A-1 (IPSC0039, LRRK2 G2385R (CBIGi027) correction) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease STBCi004-B-1 (SFC832-03-06 LRRK2WT/WT C47) Donor's gene variants: LRRK2 Donor diseases: Parkinson disease EDi001-A-2 (AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-3 (AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-4 (AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease CBIGi039-A-1 (IPSC0051, LRRK2 R1441H(CBIGi039-A) correction) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi041-A-1 (IPSC0054, GBA L444P (CBIGi041-A) correction) Donor's gene variants: GBA, GBA Donor diseases: Parkinson Disease CBIGi042-A-1 (GBA E326K (CBIGi042-A) correction, IPSC0056) Donor's gene variants: GBA, GBA Donor diseases: Parkinson Disease CBIGi044-A-1 (IPSC0059, LRRK2 N551K (CBIGi044-A) correction) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi044-A-2 (IPSC0060, LRRK2 R1398H (CBIGi044-A) correction) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease WIBRe001-A-58 (WIBR3_VPS13C_FS_Homo_H3-1) WIBRe001-A-59 (WIBR3_VPS13C_A444P_Het_E12-1) WIBRe001-A-60 (WIBR3_VPS13C_A444P_Homo_C8-2) WIBRe001-A-5 (WIBR3_PRKN_X3DEL_F2-5) WIBRe001-A-6 (WIBR3_PRKN_X3DEL_H2-2) WIBRe001-A-7 (WIBR3_SNCA_A30P_A2-3) |
| Last update | 17th October 2019 |
| User feedback | |
Provider |
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| Generator | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences (GIBH) |
| Owner | Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences (GIBH) |
| Distributors | |
| Derivation country | China |
External Databases |
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| BioSamples | SAMEA6114040 |
| Cellosaurus | CVCL_YC27 |
| Wikidata | Q93561140 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | No |
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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External Databases (Donor) |
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| BioSamples | SAMEA6114041 |
Ethics
Also have a look at the ethics information for the parental line
GIBHi002-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
GIBHi002-A.
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Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Genes | |
| Is the used vector excisable? |
Unknown |
| Absence of reprogramming vector(s)? |
Unknown |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
mTeSR™ 1
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| GATA3 |
Yes |
| GATA6 |
Yes |
| RXRG |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| SNAI2 |
Yes |
| HAND1 |
Yes |
| CDX2 |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| PAX6 |
Yes |
| SOX1 |
Yes |
| MAP2 |
Yes |
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XX
Passage number: 15
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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