Generation of a set of isogenic iPSC lines carrying all APOE genetic variants (Ɛ2/Ɛ3/Ɛ4) and knock-out for the study of APOE biology in health and disease

Summary

APOE genotype is the strongest genetic risk factor for Alzheimer's Disease (AD). The low degree of homology between mouse and human APOE is a concerning issue in preclinical models currently used to study the role of this gene in AD pathophysiology. A key objective of ADAPTED (Alzheimer's Disease Apolipoprotein Pathology for Treatment Elucidation and Development) project was to generate in vitro models that better recapitulate human APOE biology. We describe a new set of induced pluripotent stem cells (iPSC) lines carrying common APOE variants (Ɛ2, Ɛ3, and Ɛ3/Ɛ4) and a knock-out isogenic to the parental APOE Ɛ4/Ɛ4 line (UKBi011-A). Copyright © 2021 Janssen Pharmaceutica, NV. Published by Elsevier B.V. All rights reserved.

Authors Schmid B, Holst B, Clausen C, Bahnassawy L, Reinhardt P, Bakker MHM, Díaz-Guerra E, Vicario C, Martino-Adami PV, Thoenes M, Ramirez A, Flissbach K, Grezella C, Brüstle O, Peitz M, Ebneth A, Cabrera-Socorro A
Journal Stem cell research
Publication Date 2021 Feb 2;52:102180
PubMed 33556820
DOI 10.1016/j.scr.2021.102180

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