BIHi001-B-8

General

Cell Line

hPSCreg name BIHi001-B-8
Cite as:
BIHi001-B-8
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BIHi001-A
(BCRT-3, BCRT#1)
UHi006-A
(HEL24.3)
UHi006-A-1
(UHi001-A, HEL24.3-SOX2-nTdT-C9-H5, HEL24.3-SOX2-nTdT)
UHi006-A-2
(HEL24.3-OCT4-nEmGFP-A-G3, UHi001-B, HEL24.3-OCT4-nEmGFP)
UOSi001-B
(MIFF3, MIFF-3, ShiPS-MIFF3)
UOSi001-A
(MIFF1, ShiPS-MIFF1, MIFF-1)
BIHi001-A-1
(iBCRT Cas9v1-3G-Kl.16)
Last update 17th November 2023
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Provider

Generator Berlin Institute of Health (BIH)
Owner Berlin Institute of Health (BIH)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA114633139

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian, White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA103885930

Ethics

Also have a look at the ethics information for the parental line BIHi001-B .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIHi001-B.

Reprogramming method

Vector type Non-integrating
Vector single-stranded RNA replicon vector
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Essential 8™

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line BIHi001-B .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
SLC16A2 (target)
Gene knock-out
Xq13.2
NM_006517 c.1196_1196delC, p.F400Sfs*17
CRISPR-associated (CRISPR/Cas) System