BIHi001-B-9

The cell line is not validated yet.

General

Cell Line

hPSCreg name BIHi001-B-9
Cite as:
BIHi001-B-9
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 10th February 2025
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Provider

Generator Berlin Institute of Health (BIH)

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian, White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA103885930

Ethics

Also have a look at the ethics information for the parental line BIHi001-B .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIHi001-B.

Reprogramming method

Vector type Non-integrating
Vector single-stranded RNA replicon vector
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
THRA (target)
Transgene expression
17q21.1
Knock-in of the 2xHA tag upstream of THRA1 stop codon (NC_000017.10: g.38245708_38245709insCCCATACGACGTCCCAGACTACGCTTATCCATATGATGTTCCTGATTATGCATA, NP_955366.1: p.(*411Tyrext*18))
CRISPR-associated (CRISPR/Cas) System