HEL24.3-OCT4-nEmGFP-A-G3, UHi001-B, HEL24.3-OCT4-nEmGFP

General#

Cell Line

hPSCreg Name UHi006-A-2
Alternative name(s)
HEL24.3-OCT4-nEmGFP-A-G3, UHi001-B, HEL24.3-OCT4-nEmGFP
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th August 2020
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Provider

Generator University of Helsinki (UH)
Owner University of Helsinki (UH)
Distributors
Derivation country Finland

External Databases

BioSamples SAMEA104277230

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian, White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA103885930

Ethics#

Also have a look at the ethics information for the parental line UHi006-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line UHi006-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SOX2
Yes
LIN28A
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 36
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
POU5F1 (target)
Gene knock-in
T2A-NLS-EmGFP-P2A-Pac knocked in to POU5F1 stop codon
CRISPR-associated (CRISPR/Cas) System