BCRT-3, BCRT#1

General

Cell Line

hPSCreg name BIHi001-A
Cite as:
BIHi001-A (RRID:CVCL_IT54)
Alternative name(s)
BCRT-3, BCRT#1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BIHi001-A-1
(iBCRT Cas9v1-3G-Kl.16)
UHi006-A
(HEL24.3)
UOSi001-B
(MIFF3, MIFF-3, ShiPS-MIFF3)
UOSi001-A
(MIFF1, ShiPS-MIFF1, MIFF-1)
UHi006-A-1
(UHi001-A, HEL24.3-SOX2-nTdT-C9-H5, HEL24.3-SOX2-nTdT)
UHi006-A-2
(HEL24.3-OCT4-nEmGFP-A-G3, UHi001-B, HEL24.3-OCT4-nEmGFP)
Last update 25th April 2022
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Provider

Generator Berlin Institute of Health (BIH)
Owner Berlin Institute of Health (BIH)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA103885931
Cellosaurus CVCL_IT54
Wikidata Q54796557

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclones

Donor Information

General Donor Information

Sex male
Age of donor (at collection) neonate
Ethnicity Caucasian, White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA103885930

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. http://www.lgcstandards-atcc.org/en/Support/LGC_Contact_Us.aspx
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information http://www.lgcstandards-atcc.org/en/Support/LGC_Contact_Us.aspx
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name CCD-1112Sk
Other names
Derived from same source line (potentially other lot and donor, see below):
Source cell type
Dermal fibroblasts are the major cell type in dermis and are commonly accepted as terminally differentiated cells.
Source cell origin
A retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
  • prepuce
  • preputium
  • penile prepuce
  • prepuce of male
  • preputium penis
  • foreskin
  • praeputium
  • prepucium
show more synonyms
Age of donor (at collection) neonate
Source cell line vendor ATCC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Pluripotency Score Novelty Score Link to microarray data
31.32 1.5 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5145
Morphology pictures
Transcriptome Characterisation
Differentiation Potency

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: KaryoLite Bobs

Other Genotyping (Cell Line)