BCRT-3, BCRT#1

General#

Cell Line

hPSCreg Name BIHi001-A
Alternative name(s)
BCRT-3, BCRT#1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 11th June 2018
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Provider

Generator Berlin Institute of Health (BIH)
Owner Berlin Institute of Health (BIH)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA103885931
Cellosaurus CVCL_IT54

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research
Subclones

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian, White

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA103885930

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name CCD-1112Sk
Other names
Derived from same source line (potentially other lot and donor, see below):
Source cell type
foreskin fibroblast
Fibroblast from foreskin.
Synonyms
  • BJ fibroblast
Source cell origin
prepuce of penis
A retractable double-layered fold of skin and mucous membrane that covers the glans penis and protects the urinary meatus when the penis is not erect.
Synonyms
  • penile prepuce
  • prepuce of male
  • preputium penis
Age of donor (at collection) neonate
Source cell line vendor ATCC

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Pluripotency Score Novelty Score Link to microarray data
31.32 1.5 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5145
Morphology pictures
Transcriptome Characterisation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: KaryoLite Bobs

Other Genotyping (Cell Line)