Generation and characterization of a genetic Parkinson's disease-patient derived iPSC line DJ-1-delP (LCSBi008-A)
Summary
Here, we describe an induced pluripotent stem cell (iPSC) line that was derived from fibroblasts obtained from a monogenic Parkinson's disease (PD) patient. The disease was caused by a c.634-636delGCC mutation in the PARK7 gene leading to p.158P deletion in the protein DJ-1. iPSCs were generated via electroporation using three episomal plasmids encoding human Oct3/4, Sox2, Klf4, Lin28, L-Myc combined with a short hairpin RNA for p53. The presence of the c.471_473delGCC mutation in exon 7 of PARK7 was confirmed by Sanger sequencing. The iPSCs express pluripotency markers, are capable of in vitro differentiation into the three germ layers and obtain karyotypic integrity. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Mencke P, Boussaad I, Önal G, Kievit AJA, Boon AJW, Mandemakers W, Bonifati V, Krüger R |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2022 Jul;62:102792 |
| PubMed | 35500378 |
| DOI | 10.1016/j.scr.2022.102792 |