HuES4
HVRDe004-A
General#
Cell Line |
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hPSCreg Name | HVRDe004-A |
Alternative name(s) |
HuES4
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 24th August 2017 |
User feedback | |
Provider |
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Generator |
Harvard University (HVRD)
Contact:
Melton Lab |
Derivation country | United States |
External Databases |
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Cellosaurus | CVCL_B172 |
NIHhESC | NIHhESC-09-0017 |
CLO | CLO_0100162 |
General Information |
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Publications |
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Projects | |
* Is the cell line readily obtainable for third parties? |
Yes |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases |
Ethics#
Alternatives to consent are available? | Yes |
Alternatives to consent |
hESC Derivation#
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
|
PGD Embryo? |
No |
Expansion status |
3![]() |
ICM morphology |
B![]() |
Trophectoderm morphology |
b![]() |
ZP removal technique | Chemical |
Cell isolation | Immunosurgery |
Cell seeding | Isolated ICM |
Culture Conditions#
CO2 Concentration | 5 % | ||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 10 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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DNMT3B |
Yes |
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GABRB3 |
Yes |
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GDF3 |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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ZFP42 (REX-1) |
Yes |
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SOX2 |
Yes |
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TDGF1 |
Yes |
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Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 21
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Other Genotyping (Cell Line) |
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