Generation of a homozygous ABCA7 knockout cell line (AHMUCNi002-A) in human iPSCs using CRISPR/Cas9
Summary
ABCA7, located on chromosome 19, encodes an ATP-binding cassette transporter. Loss-of-function variants of ABCA7 are associated with an increased risk of Alzheimer's disease. To explore the role of ABCA7 deficiency in the pathogenesis of Alzheimer's disease, CRISPR/Cas9 genome-editing technology was utilized to generate a homozygous ABCA7 knockout in human induced pluripotent stem cells (hiPSCs). The resulting ABCA7 knockout cell line exhibited normal pluripotency, a stable karyotype, and the ability to differentiate into all three germ layers. Copyright © 2025 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Li J, Yin L, Wang C, Xu Y |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2025 Mar 25;85:103700 |
| PubMed | 40157320 |
| DOI | 10.1016/j.scr.2025.103700 |