KCL-001 (WT-3)
KCLe001-A
General
Cell Line |
|
| hPSCreg name | KCLe001-A |
| Cite as: | KCLe001-A (RRID:CVCL_A237) |
| Alternative name(s) |
KCL-001 (WT-3)
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 4th September 2022 |
| User feedback | |
Provider |
|
| Generator |
King's College London (KCL)
Contact:
Stem Cell Biology Laboratory |
| Derivation country | United Kingdom |
External Databases |
|
| Cellosaurus | CVCL_A237 |
| Wikidata | Q54899650 |
General Information |
|
| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
Yes |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
|||||||||
| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: DMEM/F12
Main protein source: Fetal bovine serum Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| Actin |
Yes |
|
||||
| FGF4 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| ZFP42 (REX-1) |
Yes |
|
||||
| SOX2 |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.