Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9


The TLR family plays a fundamental function in the recognition of pathogens and activation of innate immunity. TLR2 is a membrane protein that is expressed on the surface of certain cells and recognizes foreign substances; it initiates a cascade of innate immune responses. TLR2 recognizes many bacterial, fungal, and viral components, as well as certain endogenous substances. We generated biallelic mutants of the TLR2 gene in human induced pluripotent stem cells (hiPSCs) using a CRISPR-Cas9 genome editing method. The TLR2 heterozygous-knockout hiPSCs retained normal morphology, gene expression, and in vivo differentiation potential. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Han HJ, Kim JH
Journal Stem cell research
Publication Date 2021 Oct 19;57:102578
PubMed 34710836
DOI 10.1016/j.scr.2021.102578

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