KSCBi005-A-7
General
Cell Line |
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| hPSCreg name | KSCBi005-A-7 |
| Cite as: | KSCBi005-A-7 (RRID:CVCL_WR17) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
KSCBi005-A (CMC-hiPSC-003) KSCBi005-A-1 (CMC-hiPSC-003(Nestin-GFP), CMC-003i-Nestin.EGFP) KSCBi005-A-2 (CMC-hiPSC-003(Sox1-GFP), CMC-003i-Sox1.EGFP) KSCBi005-A-3 (CMC-003i-Pdx1.EGFP, CMC-hiPSC-003(PDX1-GFP)) |
| Last update | 27th October 2021 |
| User feedback | |
Provider |
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| Generator |
National Stem Cell Bank (KSCB)
Contact:
National Stem Cell Bank (KSCB) |
External Databases |
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| Cellosaurus | CVCL_WR17 |
| BioSamples | SAMEA9699777 |
| Wikidata | Q95980259 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA5713593 |
Ethics
Also have a look at the ethics information for the parental line
KSCBi005-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
KSCBi005-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
No characterisation data could be found for this subclone. Please open parental cell line KSCBi005-A .Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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