KSCBi005-A-7

General#

Cell Line

hPSCreg Name
KSCBi005-A-7
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 27th October 2021
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Provider

Generator National Stem Cell Bank (KSCB)
Distributors

External Databases

Cellosaurus CVCL_WR17
BioSamples SAMEA9699777
Wikidata Q95980259
CLO CLO_0103538

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA5713593

Ethics#

Also have a look at the ethics information for the parental line KSCBi005-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line KSCBi005-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line KSCBi005-A

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
(unknown disease)
Genetic modifications
Gene knock-out
4q31.3
Please explain briefly the supporting evidence
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease
Gene knock-out
4q31.3
Please explain briefly the supporting evidence
CRISPR-associated (CRISPR/Cas) System