CMC-hiPSC-003(Sox1-GFP), CMC-003i-Sox1.EGFP
KSCBi005-A-2
General
Cell Line |
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| hPSCreg name | KSCBi005-A-2 |
| Cite as: | KSCBi005-A-2 (RRID:CVCL_WR12) |
| Alternative name(s) |
CMC-hiPSC-003(Sox1-GFP), CMC-003i-Sox1.EGFP
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
KSCBi005-A (CMC-hiPSC-003) KSCBi005-A-1 (CMC-hiPSC-003(Nestin-GFP), CMC-003i-Nestin.EGFP) KSCBi005-A-3 (CMC-003i-Pdx1.EGFP, CMC-hiPSC-003(PDX1-GFP)) |
| Last update | 6th August 2019 |
| User feedback | |
Provider |
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| Generator | National Stem Cell Bank (KSCB) |
| Owner | National Stem Cell Bank (KSCB) |
| Distributors | |
| Derivation country | South Korea |
External Databases |
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| BioSamples | SAMEA5837725 |
| Cellosaurus | CVCL_WR12 |
| Wikidata | Q95980254 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
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External Databases (Donor) |
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| BioSamples | SAMEA5713593 |
Ethics
Also have a look at the ethics information for the parental line
KSCBi005-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
KSCBi005-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| Medium |
TeSR™ E8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| GDF3 |
Yes |
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| TERT |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| Alkaline Phosphatase |
Yes |
Morphology pictures
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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