KSCBi005-A-1

CMC-hiPSC-003(Nestin-GFP), CMC-003i-Nestin.EGFP

General#

Cell Line

hPSCreg Name KSCBi005-A-1
Alternative name(s)
CMC-hiPSC-003(Nestin-GFP), CMC-003i-Nestin.EGFP
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 22nd May 2019
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Provider

Generator National Stem Cell Bank (KSCB)
Owner National Stem Cell Bank (KSCB)
Distributors
Derivation country South Korea

External Databases

BioSamples SAMEA5713482
Cellosaurus CVCL_WR11

General Information

Notes Nestin-EGFP reporter human induced pluripotent stem cell line
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA5713593

Ethics#

Also have a look at the ethics information for the parental line KSCBi005-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line KSCBi005-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection All transgenes were silenced.
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
TRA 1-81
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
TERT
Yes
GDF3
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
MYOG
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
PAX6
Yes
SOX1
Yes
NES
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
Gene knock-in
1q23.1
1q23.1
CRISPR-associated (CRISPR/Cas) System