Generation of a NESTIN-EGFP reporter human induced pluripotent stem cell line, KSCBi005-A-1, using CRISPR/Cas9 nuclease


NESTIN, an intermediate filament, is a neuroectodermal marker involved in induced pluripotent stem cell (iPSC) differentiation toward neural lineages. Here, we introduced an EGFP reporter into the C-terminus of NESTIN in KSCBi005-A hiPSCs through homologous recombination using CRISPR/Cas9 nuclease. The successfully edited line was confirmed by sequencing and had a normal karyotype. It expressed EGFP upon induction of neural differentiation and exhibited potential for differentiation into three germ layers. KSCBi005-A-1 cells could be used to monitor the expression of NESTIN in differentiated cell types. This cell line is available at the National Stem Cell Bank, Korea National Institute of Health. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Lee Y, Choi HY, Kwon A, Park H, Park M, Kim YO, Kwak S, Koo SK
Journal Stem cell research
Publication Date 2019 Oct;40:101554
PubMed 31499408
DOI 10.1016/j.scr.2019.101554

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