In vitro germ cell induction from fertile and infertile monozygotic twin research participants


Human induced pluripotent stem cells (hiPSCs) enable reproductive diseases to be studied when the reproductive health of the participant is known. In this study, monozygotic (MZ) monoamniotic (MA) twins discordant for primary ovarian insufficiency (POI) consent to research to address the hypothesis that discordant POI is due to a shared primordial germ cell (PGC) progenitor pool. If this is the case, reprogramming the twin's skin cells to hiPSCs is expected to restore equivalent germ cell competency to the twins hiPSCs. Following reprogramming, the infertile MA twin's cells are capable of generating human PGC-like cells (hPGCLCs) and amniotic sac-like structures equivalent to her fertile twin sister. Using these hiPSCs together with genome sequencing, our data suggest that POI in the infertile twin is not due to a genetic barrier to amnion or germ cell formation and support the hypothesis that during gestation, amniotic PGCs are likely disproportionately allocated to the fertile twin with embryo splitting. Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Authors Pandolfi EC, Hsu FM, Duhon M, Zheng Y, Goldsmith S, Fu J, Silber SJ, Clark AT
Journal Cell reports. Medicine
Publication Date 2022 Oct 18;3(10):100782
PubMed 36260988
PubMed Central PMC9589117
DOI 10.1016/j.xcrm.2022.100782

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