Reprogramming Patient-Derived urine cells into iPSCs for Anti-GAD65 autoimmune encephalitis research
Summary
Urine cells from a patient with anti-GAD65autoantibody-associated autoimmune limbic encephalitis (ALE) were reprogrammed intoiPSCline IUFi020-A. Pluripotency was confirmed throughhiPSCore analysis while G-banding and CNVanalysis demonstrated that IUFi020-A exhibits characteristic features of a bona fide iPSC line with no genetic changes compared to the donor urine cells. STRanalysis further confirmed that IUFi020-A was derived from the parental urine cells. Additional characterization verified the absence of plasmidintegration and mycoplasma contamination.IUFi020-Aline provides a non-invasive alternative to fibroblast-based reprogramming and serves as a valuable model for investigating the pathogenic mechanisms of anti-GAD65-associated ALE. Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ramachandran H, Räuber S, Dobner J, Hildebrandt B, Haslinger D, Chiocchetti AG, Disse P, Preuth LM, Therpurakal RN, Meuth SG, Melzer N, Rossi A |
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| Journal | Stem cell research |
| Publication Date | 2025 Jul 28;87:103790 |
| PubMed | 40752256 |
| DOI | 10.1016/j.scr.2025.103790 |