ES-4
ESe014-A
General#
Cell Line |
|
| hPSCreg Name | ESe014-A |
| Alternative name(s) |
ES-4
|
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
|
| Generator | Spanish Stem Cell Bank (ES) |
| Derivation country | Spain |
External Databases |
|
| BioSamples | SAMEA7778668 |
| Cellosaurus | CVCL_C771 |
General Information |
|
| Publications |
|
| Projects | |
| Is the cell line available in principle? |
Yes |
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
External Databases (Donor) |
|
| BioSamples | SAMEA7777918 |
Ethics#
We are currently revising this section.hESC Derivation#
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
1
|
| ICM morphology |
D
|
| Trophectoderm morphology |
c
|
| ZP removal technique | Chemical |
| Cell isolation | None |
| Cell seeding | Embryo |
Culture Conditions#
| Surface coating | Gelatin | |||||||||
| Feeder cells |
CRL-2429 Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419 |
|||||||||
| O2 Concentration | 21 % | |||||||||
| CO2 Concentration | 5 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| Telomerase Activity |
Yes |
|
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XY
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.