ES-4, ES[4]
ESe014-A
General#
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | |
External Databases (Donor) |
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BioSamples | SAMEA7777918 |
Ethics#
Was the embryo established purely for research purposes? | No |
Have both parents consented to the use of the embryo for ESC derivation? | Yes |
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Alternative consent approval number | |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
How may genetic information associated with the cell line be accessed? | |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Ethics Board of the Center of Regenerative Medicine in Barcelona |
Approval number |
hESC Derivation#
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
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PGD Embryo? |
No |
Expansion status |
1![]() |
ICM morphology |
D![]() |
Trophectoderm morphology |
g![]() |
ZP removal technique | Chemical |
Cell isolation | None |
Cell seeding | Embryo |
Culture Conditions#
Surface coating | Gelatin | |||||||||
Feeder cells |
CRL-2429 Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419 |
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O2 Concentration | 21 % | |||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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SOX2 |
Yes |
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SSEA-1 |
No |
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Telomerase Activity |
Yes |
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Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
Passage number: 22
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Other Genotyping (Cell Line) |
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