SA002
CEBe034-A
General
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | NIHhESC-10-0086 |
hESC Derivation
| Date of derivation | 2001-05-21 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Spontaneous Hatching |
| Cell isolation | None |
| Cell seeding | Embryo |
Culture Conditions
| Medium |
VitroHES
Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
|
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| TRA 1-81 |
Yes |
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| hES-Cellect |
Yes |
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| SSEA-1 |
No |
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| Telomerase Activity |
Yes |
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| NANOG |
Yes |
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| DNMT3B |
Yes |
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| GABRB3 |
Yes |
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| TDGF1 |
Yes |
|
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
47XX,+13
Passage number: 25
|
Other Genotyping (Cell Line) |
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