SA002

General

Cell Line

hPSCreg name CEBe034-A
Cite as:
CEBe034-A (RRID:CVCL_B348)
Alternative name(s)
SA002
Cell line type Human embryonic stem cell (hESC)
Similar lines
CEBe034-A-1
(SA002.5)
Donor diseases:
Patau syndrome
MIZMe015-A
(Miz-hES13)
Donor diseases:
Patau syndrome
GZHMCe001-A
(FY-hES-5)
Donor diseases:
Patau syndrome
Last update 28th July 2017
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Provider

Generator Takara Bio Europe AB (former Cellartis) (CEB)
Derivation country Sweden

External Databases

Cellosaurus CVCL_B348
NIHhESC NIHhESC-10-0086
Wikidata Q54951990

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Subclones

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • D1 Trisomy
  • trisomy 13
  • Patau's syndrome

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number NIHhESC-10-0086

hESC Derivation

Date of derivation 2001-05-21
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Spontaneous Hatching
Cell isolation None
Cell seeding Embryo

Culture Conditions

Medium VitroHES
Supplements
hrbFGF %
Serum Replacement %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
hES-Cellect
Yes
SSEA-1
No
Telomerase Activity
Yes
NANOG
Yes
DNMT3B
Yes
GABRB3
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XX,+13
Passage number: 25

Other Genotyping (Cell Line)