SA002

General#

Cell Line

hPSCreg Name CEBe034-A
Alternative name(s)
SA002
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Cellartis AB Sweden (CEB)
Derivation country Sweden

External Databases

Cellosaurus CVCL_B348
NIHhESC NIHhESC-10-0086

General Information

Publications
Projects
Is the cell line available in principle?
Yes
Subclones

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Patau syndrome
The donor is affected.
Synonyms
  • trisomy 13
  • D1 Trisomy

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2001-05-21
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Spontaneous Hatching
Cell isolation None
Cell seeding Embryo

Culture Conditions#

Medium VitroHES
Supplements
hrbFGF %
Serum Replacement %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
hES-Cellect
Yes
SSEA-1
No
Telomerase Activity
Yes
NANOG
Yes
DNMT3B
Yes
GABRB3
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
47XX,+13

Other Genotyping (Cell Line)