General

Cell Line
hPSCreg name	MIZMe015-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	MIZMe015-A (RRID:CVCL_9266)
Alternative name(s)	Miz-hES13
Cell line type	Human embryonic stem cell (hESC)
Similar lines	CEBe034-A (SA002) Donor diseases: Patau syndrome GZHMCe001-A (FY-hES-5) Donor diseases: Patau syndrome CEBe034-A-1 (SA002.5) Donor diseases: Patau syndrome
Last update	31st August 2022
User feedback show/hide	Written by on Delete No feedback available yet. Login to share your feedback, experiences or results with the research community.
Provider
Generator	MizMedi Hospital (MIZM) Contact: Medical Research Center
Derivation country	South Korea
External Databases
Cellosaurus	CVCL_9266
Wikidata	Q54905783
General Information
Publications	Kim SJ et al. Efficient derivation of new human embryonic stem cell lines. Molecules and cells. 2005 Feb 28;19(1):46-53.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Patau syndrome The donor is affected. Synonyms D1 Trisomy trisomy 13 Patau's syndrome

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	No Other hESC source: IVF
ZP removal technique	Enzymatic
Cell isolation	Immunosurgery

Culture Conditions

Feeder cells	mouse embryonic fibroblast cells Cellfinder Ont Id: EFO_0004040
Passage method	Mechanically
Medium	Other medium: Base medium: DMEM/F-12 Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit L-Glutamine hrbFGF

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
POU5F1 (OCT-4)	Yes
SSEA-1	No
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vivo teratoma

In vitro spontaneous differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vivo teratoma

In vitro spontaneous differentiation

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vivo teratoma

In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 47XX,+13 Passage number: 16
Other Genotyping (Cell Line)