FY-hES-5
GZHMCe001-A
General
Cell Line |
|
| hPSCreg name | GZHMCe001-A |
| Cite as: | GZHMCe001-A (RRID:CVCL_9925) |
| Alternative name(s) |
FY-hES-5
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
|
| Last update | 3rd September 2022 |
| User feedback | |
Provider |
|
| Generator | The Third Affiliated Hospital of Guangzhou Medical University (GZHMC) |
| Derivation country | China |
External Databases |
|
| Cellosaurus | CVCL_9925 |
| Wikidata | Q54835322 |
General Information |
|
| Publications |
|
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
| Disease associated phenotypes |
|
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| ZP removal technique | Enzymatic |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | MEF | |||||||||
| Feeder cells |
mouse embryonic fibroblast cells Cellfinder Ont Id: EFO_0004040 |
|||||||||
| Passage method | Mechanically | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| ZFP42 (REX-1) |
Yes |
|
||||
| LEFTY1 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| TDGF1 |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
47XX,+13,der(13;13)(q10;q10)
Passage number: 40
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.