Development and characterization of two independent integration free iPSC clones NCCSi010-A and NCCSi010-B from a patient with alcoholic liver cirrhosis of Indian ethnicity


We generated two human induced pluripotent stem cell-line (iPSC) clones, NCCSi010-A and NCCSi010-B, from a 32-year-old alcoholic cirrhosis patient with minimal hepatic encephalopathy of Indian origin by reprogramming his CD4+ T cells with integration free Sendai viral vector system. The generated iPSC clones showed high alkaline phosphatase activity, expressed pluripotency markers, possessed potential for multi-lineage differentiation and exhibited a normal karyotype (46, XY). These two-patient specific iPSC clones of alcoholic liver cirrhosis can potentially serve as models for disease modeling, drug development and organoid generation (Shah and Bataller, 2016). Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Vaidyanath A, Khan M, Vaishnav B, Kakrani AL, Patil S, Shiras A
Journal Stem cell research
Publication Date 2020 Jul 20;47:101911
PubMed 32745713
DOI 10.1016/j.scr.2020.101911

Research Projects

Cell Lines