CH-ES1
UNIGEe001-A
General
Donor Information
General Donor Information |
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Phenotype and Disease related information (Donor) |
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| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | National |
| Approval number |
hESC Derivation
| Date of derivation | 2007-01-01 |
| Embryo stage | Cleavage (Mitosis) |
| PGD Embryo? |
No |
| Cell isolation | Mechanical |
| Cell seeding | Single cell and ICM isolated |
Culture Conditions
| Surface coating | Human foreskin fibroblasts |
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Serum concentration: % |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-1 |
No |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-81 |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
61XX
Passage number: 40
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Other Genotyping (Cell Line) |
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