General

Cell Line

hPSCreg name QBRIi006-B
Cite as:
QBRIi006-B (RRID:CVCL_YB73)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
QBRIi006-A
Donor diseases:
psoriasis
QBRIi006-C
Donor diseases:
psoriasis
QBRIi005-A
Donor diseases:
Psoriasis
QBRIi005-B
Donor diseases:
Psoriasis
QBRIi005-C
Donor diseases:
Psoriasis
DMBi007-A
Donor diseases:
Psoriasis
DMBi008-A
Donor diseases:
Psoriasis
ASGRCi002-A
(F3-3#1 CHR)
Donor diseases:
Type 1 Diabetes Mellitus
SHFDi001-A
(SHFD-1)
Donor diseases:
Ankylosing Spondylitis
SPHi001-A
(SLEi002HZY)
Donor diseases:
Systemic Lupus Erythematosus
BIHi002-A
Donor's gene variants:
CLCN7, CLCN7
Donor diseases:
autosomal recessive osteopetrosis 4
ASGRCi003-A
(F3-4#5 CHR)
Donor diseases:
Type 1 Diabetes Mellitus
Last update 14th April 2022
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Provider

Generator Qatar Biomedical Research Institute (QBRI)
Owner Qatar Biomedical Research Institute (QBRI)
Derivation country Qatar

External Databases

BioSamples SAMEA6260531
Cellosaurus CVCL_YB73
Wikidata Q98128755

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Psoriasis
The donor is not a carrier of a disease-associated mutation and affected.
Synonyms
  • PITYRIASIS NEC & NOS
  • PUSTULOSIS OF PALMS SOLES
  • Psoriasis and similar disorders (disorder)
  • PUSTULAR PSORIASIS OF PALMS SOLES
  • OTHER PSORIASIS
  • Pustulosis of Palms and Soles
  • Psoriasis and similar disorders
  • Palmoplantaris Pustulosis
  • Pustulosis Palmaris et Plantaris
  • PSORIAS RELATED DIS NEC
  • Other psoriasis and similar disorders excluding psoriatic arthropathy
  • Psoriasis and similar disorders (navigational concept)
  • Pustular Psoriasis of Palms and Soles
  • psoriasis
  • Psoriases
  • Other psoriasis and similar disorders (disorder)
  • Other psoriasis and similar disorders
  • Psoriasis and similar disorders NOS
  • Psoriasis and similar disorders NOS (disorder)
  • Other and unspecified pityriasis
show more synonyms
Family history Family history with psoriasis

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6260529

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Dr. Mohamed Bashir (MBashir@hamad.qa)
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information Dr. Mohamed Bashir (MBashir@hamad.qa)
Alternatives to consent are available? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? No
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Institutional Review Board (IRB) Committees of HMC and Qatar Biomedical Research Institute (QBRI)
Approval number no. 16260/16 & no. 2016-003
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Institutional Review Board (IRB) Committees of HMC and Qatar Biomedical Research Institute (QBRI)
Approval number no. 16260/16 & no. 2016-003
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Mononuclear cells collected from peripheral blood.
Synonyms
  • Peripheral Blood Mononuclear cells
  • Derived Peripheral Blood Mononuclear Cell

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Yes
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
POU5F1 (OCT-4)
Yes
NANOG
Yes
Self-renewal
Unknown
Endoderm
Unknown
Mesoderm
Unknown
Ectoderm score
Positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
In vitro directed differentiation
Scorecard

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY[cp20]
Passage number: 25

Other Genotyping (Cell Line)